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biorad mini transblot cell  (Bio-Rad)


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    Structured Review

    Bio-Rad biorad mini transblot cell
    Biorad Mini Transblot Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biorad+mini+transblot+cell/Mini+Trans-Blot+Module/pm40616061-186-13-13
    Average 96 stars, based on 550 article reviews
    biorad mini transblot cell - by Bioz Stars, 2026-09
    96/100 stars

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    Membrane:

    Article Title: Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways.
    Article Snippet: .. The resolved proteins were transferred to a 0.22 μm nitrocellulose membrane by liquid transfer using Tris/Glycine buffer and BioRad Mini TransBlot Cell for 3 h at 80 V and 160 mA. .. Total proteins on the membrane were stained by incubation in Ponceau S solution (Sigma, #P7170) for 3–5 min.

    Article Title: Immunohistochemical localization of active caspase-3 in the mouse ovary: growth and atresia of small follicles.
    Article Snippet: Thirty micrograms of protein was loaded on to a 10% (w/v) SDS-polyacrylamide gel and separated under reducing conditions using a BioRad MiniProtean II unit. .. Proteins were electrotransferred with a BioRad Mini TransBlot cell on to a nitrocellulose membrane, blocked with 3% (w/v) non-fat milk powder and 0.05% (v/v) Tween 20, and probed with 1:100 anti-caspase-3 polyclonal antibody (Santa Cruz Biotech, Santa Cruz, CA) for 3 h at room temperature. .. Membranes were incubated with an anti-goat horseradish peroxidase conjugated secondary antibody (Sigma) and developed with ECL chemiluminescence according to the manufacturer’s instructions (Amersham).

    Article Title: PARP inhibitor olaparib induces DNA damage and acts as a drug sensitizer in an in vitro model of canine hematopoietic cancer.
    Article Snippet: Then, after centrifuging at 10,000 rpm at 4 °C for 12 min SDS sample buffer was added to clear the supernatants and the samples were boiled at 95 °C for 5 min and subjected to SDS-polyacrylamide gel electrophoresis in a 12% gel (BioRad Mini-PROTEAN Tetra Vertical Electrophoresis Cell system, Hercules, USA). .. After the electrophoresis, the samples were transferred to a nitrocellulose membrane using a BioRad Mini TransBlot® Cell for wet transfer and Western Blot enhancer treatment was performed according to manufacturer’s protocol with PierceTM Western Blot Signal Enhancer (Thermo Scientific). .. Then, the membranes were blocked with 3% BSA in TBST at room temperature for 1 h. After blocking, the membranes were incubated overnight at 4 °C with the following murine monoclonal primary antibodies: anti-γH2A.X clone 9F3 (ab26350; Abcam, Cambridge, United Kingdom), dilution 1:1000 and anti-β-actin clone C4 (sc47 778; Santa Cruz, California, USA), dilution 1:2000.

    Electrophoresis:

    Article Title: PARP inhibitor olaparib induces DNA damage and acts as a drug sensitizer in an in vitro model of canine hematopoietic cancer.
    Article Snippet: Then, after centrifuging at 10,000 rpm at 4 °C for 12 min SDS sample buffer was added to clear the supernatants and the samples were boiled at 95 °C for 5 min and subjected to SDS-polyacrylamide gel electrophoresis in a 12% gel (BioRad Mini-PROTEAN Tetra Vertical Electrophoresis Cell system, Hercules, USA). .. After the electrophoresis, the samples were transferred to a nitrocellulose membrane using a BioRad Mini TransBlot® Cell for wet transfer and Western Blot enhancer treatment was performed according to manufacturer’s protocol with PierceTM Western Blot Signal Enhancer (Thermo Scientific). .. Then, the membranes were blocked with 3% BSA in TBST at room temperature for 1 h. After blocking, the membranes were incubated overnight at 4 °C with the following murine monoclonal primary antibodies: anti-γH2A.X clone 9F3 (ab26350; Abcam, Cambridge, United Kingdom), dilution 1:1000 and anti-β-actin clone C4 (sc47 778; Santa Cruz, California, USA), dilution 1:2000.

    Western Blot:

    Article Title: PARP inhibitor olaparib induces DNA damage and acts as a drug sensitizer in an in vitro model of canine hematopoietic cancer.
    Article Snippet: Then, after centrifuging at 10,000 rpm at 4 °C for 12 min SDS sample buffer was added to clear the supernatants and the samples were boiled at 95 °C for 5 min and subjected to SDS-polyacrylamide gel electrophoresis in a 12% gel (BioRad Mini-PROTEAN Tetra Vertical Electrophoresis Cell system, Hercules, USA). .. After the electrophoresis, the samples were transferred to a nitrocellulose membrane using a BioRad Mini TransBlot® Cell for wet transfer and Western Blot enhancer treatment was performed according to manufacturer’s protocol with PierceTM Western Blot Signal Enhancer (Thermo Scientific). .. Then, the membranes were blocked with 3% BSA in TBST at room temperature for 1 h. After blocking, the membranes were incubated overnight at 4 °C with the following murine monoclonal primary antibodies: anti-γH2A.X clone 9F3 (ab26350; Abcam, Cambridge, United Kingdom), dilution 1:1000 and anti-β-actin clone C4 (sc47 778; Santa Cruz, California, USA), dilution 1:2000.



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    96
    Bio-Rad biorad mini transblot cell
    Biorad Mini Transblot Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biorad+mini+transblot+cell/Mini+Trans-Blot+Module/pm40616061-186-13-13
    Average 96 stars, based on 1 article reviews
    biorad mini transblot cell - by Bioz Stars, 2026-09
    96/100 stars
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